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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in many tumors, which results in chromosome instability, and so may add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a significant job in advertising and marketing cell-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have already been explained. Two added splice variants were claimed but specific nucleotide sequence details is not nevertheless accessible.
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Blocking peptides are peptides that bind especially to the target antibody and block antibody binding. These peptide typically has the epitope recognized because of the antibody.
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This manual outlines further probable optimization of cellular and immunoassay parameters to be certain the best possible benefits are...
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This guidebook outlines even more attainable optimization of mobile and immunoassay parameters to make certain the best possible benefits are...
Antibodies sure to the blocking peptide now not bind to the get more info epitope on the concentrate on protein. By evaluating the staining from the blocked antibody as opposed to the antibody by itself, one can see which staining is specific.
Goal Information Cyclin E belongs for the hugely conserved cyclin family, whose members are characterized by a spectacular periodicity in protein abundance from the cell cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of every mitotic occasion. Cyclin E types a posh with and functions as being a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells progress by S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both equally antibodies...
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